Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.
نویسندگان
چکیده
منابع مشابه
Large-scale purification and acute toxicity of hygromycin B phosphotransferase.
OBJECTIVE To provide the acute toxicity data of hygromycin B phosphotransferase (HPT) using recombinant protein purified from E. coli. METHODS Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared ...
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A hygromycin B phosphotransferase activity from Streptomyces hygroscopicus has been highly purified by ammonium sulphate fractionation followed by affinity column chromatography through Sepharose-6B-hygromycin-B. The combined active fractions showed a single protein band (41 kDa) when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. When gel electropho...
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We have tested whether the thymidine kinase gene of herpes simplex virus 1 (HSV-1 tk) could function as a negative selectable marker in Leishmania major. Several drug resistance markers have been used for positive selection of transfected DNA in trypanosomatids, including neomycin phosphotransferase (NEO; refs. 1-4) and hygromycin phosphotransferase (HYG; refs. 5,6). Negative selectable markers...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1984
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.4.12.2929